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The use of agar-agar represents one of the most fundamental and essential techniques in modern mycology, offering mycologists and growers a robust and sterile medium for the propagation and study of fungal mycelium. This article explores the most advanced methodologies for maximizing the potential of this extraordinary polysaccharide, with detailed recipes, sterilization protocols, and inoculation techniques that ensure successful propagation of the most valuable fungal strains. Through this comprehensive guide, we will explore every aspect of the preparation and use of agar-based culture media, providing scientific data, detailed statistics, and proven procedures to optimize results in mushroom cultivation at both a professional and amateur level.

Mushroom cultivation represents one of the most fascinating frontiers of applied mycology, a bridge between cutting-edge scientific research and practical production techniques. Among the factors determining the success or failure of a cultivation, the composition of the growth medium plays a fundamental role. In this article, we will explore in depth the structure of lignocellulose, analyze the delicate balance between cellulose and lignin, and discover how this ratio directly influences the ability of fungi to colonize the substrate, with implications ranging from production yield to biomass degradation efficiency.

Substrate hydration is one of the key parameters in the mushroom cultivation process, an aspect that is too often overlooked or approached with approximate methodologies. In this in-depth technical article, we will explore in detail the concepts of water holding capacity (WHC) and field capacity, two essential parameters for ensuring proper substrate hydration and maximizing production yields in mycelial cultivation. Through scientific data, comparative tables, and detailed analyses, we will provide mycelium growers and mycology enthusiasts with all the tools necessary to master this crucial phase of the cultivation process.